Fluoromount-G Mounting Medium
- Prepare cytocentrifuge preps according to established procedures.
- Fix and rehydrate preps.
- Remove one slide at a time from aqueous buffer, add one (1) drop of Fluoromount-GT directly to cell prep, mount cover slip and press gently with a gauze sponge to remove excess mounting medium and to seal the cover slip.
- Allow mounted preps to air-dry for five (5) minutes before examination.
Handling And Storage:
Fluoromount-GT is supplied as a 25 ml solution containing 0.1% sodium azide as preservative; store at room temperature.
Removal of Fluoromount™
Fluoromount™ is an aqueous based mounting medium and can easily be removed by soaking the slides in deionized water:
- Place the slide in a beaker full of deionized water on a magnetic stirrer.
- Leave the slide for few hours to overnight with gentle stirring for complete removal of dry Fluoromount
Reagents contain sodium azide, which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Fluoromount-G Mounting Medium is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. For convenience, this medium is provided with and without DAPI, a blue nuclear counter stain. It is water soluble and used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C. Fluoromount-G Mounting Medium does not fluoresce and may reduce the amount of fluorochrome quenching and photobleaching during fluorescence microscopy. This medium is supplied as a 1X stock solution containing sodium azide. The refractive index of Fluoromount-G Mounting Medium is reported to be ∼1.4, and it is best used when the thickness of the specimen is less than 10 µm.