Exogenetic overexpression of PRIM2 rescued the inhibitory effects of DHA on proliferation and colony formation in lung cancer cells. (A) Overexpression of PRIM2 was confirmed by Western blot assay.
(B-D) Cell viability and colony-forming ability of NCI-H23 cells were detected by CCK-8 assay and colony-forming assay. (E-G) Measurement of cellular GSH, cellular ROS, and mitochondrial MDA in NCI-H23 cells that were treated with 60 μM DHA and transfected with the PRIM2 OE plasmid. (H) Protein expressions of SLC7A11 and β-catenin were analyzed by Western Blot. The experiments were repeated three times.
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DNA replication in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of small subunit and large subunit, synthesizes small RNA primers for Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the 58 kDa large subunit primase.
This gene codes for the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in DNA replication. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers which are used to create Okazaki fragments on the lagging strand of DNA. Alternative splicing of this gene results in multiple transcription variants. This gene has a related pseudogene, which is also present on chromosome 6.
Small volumes of anti-PRIM2 antibody vial(s) may sometimes become stuck in the seal of the product vial during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the container cap. Some products may need to be shipped with dry ice and additional dry ice charges may apply.