Description
Chimeric Anti EBOV (C6D8) | 494-0201-021
Catalog #:
0201-021
Type:
Antibody Monoclonal
Specific against:
other
Amount:
100ug
Host:
Mouse
mmunogen:
Venezuelan equine encephalitis virus replicons encoding Zaire ebolavirus (ZEBOV) glycoprotein (GP) was used to generate the original mouse monoclonal antibody.
Description:
A murine / human chimeric IgG produced in N. benthamiana and is reactive to ZEBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant GP without the transmembrane region (rGPdTM).
Supplied:
100 µg is supplied at a concentration of 3.85 mg/mL. No preservative is added.
Purification:
Antibody is purified using immobilized protein A.
Clonality:
Murine variable, human constant of the IgG1 isotype.
Relevance:
The antibody can be used for detection of ZEBOV GP. A mixture of all three anti-ZEBOV GP chimeric antibodies was protective against lethal challenge in a nonhuman primate study (Olinger et al. PNAS 2012, vol. 109, no. 44, 18030-18035).
ELISA:
Assay-dependent dilution
WB:
Assay-dependent dilution. Internal QC demonstrates good detection when using antibody at 0.5 µg/mL.
Cross Reactivity:
No cross-reactivity was observed to Sudan ebolavirus (SEBOV) or Marburg Virus (MARV) VLP or rGPdTM
Storage:
2-3 weeks +4oC, -20◦C long term
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